Video 4.1 GFP-VAMP8 trafficking from a late endosome to the cell surface (54-81 sec).

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RAW264.7 macrophages were transiently transfected with GFP-VAMP8 and LAMP1-mCherry. Cells were stimulated with LPS (100 ng/ml) for 6 hrs, then imaged by live epifluorescence microscopy capturing an image every 2-4 sec for 3 min.